C3a ELISA Kit

Produktnummer ABIN8068488

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human C3a.

Kurzübersicht für C3a ELISA Kit (ABIN8068488)

Target: C3a (Complement Fragment 3a (C3a))

Bindungsspezifität: carbamylated, total

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Cell Lysate, Tissue Lysate

Produktdetails C3a ELISA Kit

Verwendungszweck: Measures carbamylated C3a and total C3a in human cell and tissue lysates, enabling precise assessment of C3a carbamylation by ELISA. This assay can be developed for other species (mouse, rat, etc.) upon request. Contact techsupport@raybiotech.com.

Analytische Methode: Quantitative

Bestandteile:

Size / Qty
• Microplate Coated With Anti-Pan-C3a Antibody
• Anti-Carbamylation Detection Antibody
• Anti-C3a Detection Antibody
• HRP-Conjugated Streptavidin
• 200 μL
• Wash Buffer
• Assay Diluent
• Lysis Buffer
• TMB One-Step Substrate Reagent
• Stop Solution

Benötigtes Material:

• Microplate reader capable of measuring absorbance at 450 nm
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Log-log graph paper or computer and software for ELISA data analysis
• Absorbent paper
• Tubes to prepare sample dilutions
• Distilled or deionized water
• Shaker

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents and samples as instructed.
2. Add 100 μL sample to each well. Incubate 2.5 hours at room temperature or overnight at 4 °C with gentle shaking.
3. Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
4. Add 100 μL prepared HRP-Conjugated Streptavidin solution. Incubate for 1 hour at room temperature with gentle shaking.
5. Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
6. Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Details zu C3a

Target: C3a (Complement Fragment 3a (C3a))

Andere Bezeichnung: C3a

Hintergrund:

Gene Symbols: C3, CPAMD1

Background: The Carbamylated-C3a and Total-C3a ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human carbamylated ATP-citrate synthase and total ATP-citrate synthase. An anti-pan C3a antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and C3a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-carbamylation antibody is used to detect carbamylated C3a or biotinylated anti-C3a antibody is used to detect total C3a . After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of carbamylated C3a or total C3a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Protein Name (Synonyms): Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

Gen-ID: 718

UniProt: P01024

Pathways: Komplementsystem