c-MET ELISA Kit

Produktnummer ABIN8055957

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Hund c-MET.

Kurzübersicht für c-MET ELISA Kit (ABIN8055957)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Reaktivität: Hund

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.0 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails c-MET ELISA Kit

Untere Nachweisgrenze: 156.0 pg/mL

Verwendungszweck: Sandwich Canine HGFR ELISA Kit to quantitate Canine HGFR in cell culture supernatants, serum, plasma(heparin, EDTA).

Analytische Methode: Quantitative

Spezifität:

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 7.9%, 3.5%, 5.3%
Inter-Assay Precision (CV%): 5.4%, 5.7%, 7.2%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu c-MET

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Andere Bezeichnung: MET

Hintergrund:

Background: Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. Enables hepatocyte growth factor receptor activity. Involved in cell surface receptor signaling pathway, modulation of chemical synaptic transmission, and positive chemotaxis. Acts upstream of or within several processes, including hepatocyte growth factor receptor signaling pathway, regulation of cytokine production, and striated muscle cell differentiation. Located in membrane. Is expressed in several structures, including alimentary system, branchial arch, central nervous system, genitourinary system, and limb.

Gene Full Name: Hepatocyte growth factor receptor

Gen-ID: 403438

UniProt: Q75ZY9

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor