BMP6 ELISA Kit

Produktnummer ABIN8055908

Human BMP6 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human BMP6.

Kurzübersicht für BMP6 ELISA Kit (ABIN8055908)

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156.0 pg/mL - 10000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Osseous Tissue, Serum

Produktdetails BMP6 ELISA Kit

Untere Nachweisgrenze: 156.0 pg/mL

Verwendungszweck: Sandwich Human BMP-6 ELISA Kit to quantitate Human BMP-6 in Bone tissue, cell culture supernatants, serum.

Analytische Methode: Quantitative

Spezifität:

Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 20 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 3.8%, 5.1%, 7.3%
Inter-Assay Precision (CV%): 2.2%, 4.2%, 2.0%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu BMP6

Target: BMP6 (Bone Morphogenetic Protein 6 (BMP6))

Andere Bezeichnung: BMP6

Hintergrund:

Background: The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, BMP-6 has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has lead to speculation of possible bone inductive activity.

Gene Full Name: bone morphogenetic protein 6

Gen-ID: 654

UniProt: P22004

Pathways: Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process