C1R ELISA Kit

Produktnummer ABIN8055520

Das Human C1R ELISA Kit (ABIN8055520) ist ein Colorimetric ELISA Kit zur Detektion von Human C1R.

Kurzübersicht für C1R ELISA Kit (ABIN8055520)

Target: C1R (Complement Component 1, R Subcomponent (C1R))

Bindungsspezifität: AA 18-705

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 1.56 ng/mL - 100 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

Produktdetails C1R ELISA Kit

Untere Nachweisgrenze: 1.56 ng/mL

Verwendungszweck: Sandwich Human Complement C1R ELISA Kit to quantitate Human C1R in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) and urine.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: S18-D705

Capture antibody and Detection antibody: a monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 0.1 ng/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 4.8%, 5.2%, 4.6%
Inter-Assay Precision (CV%): 6.1%, 5.9%, 6.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu C1R

Target: C1R (Complement Component 1, R Subcomponent (C1R))

Andere Bezeichnung: C1R

Hintergrund:

Background: Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. It is mapped to 12p13.31. In humans, C1r is encoded by the C1R gene. C1r along with C1q and C1s form the C1 complex, which is the first component of the serum complement system. C1r is an enzyme that activates C1s to its active form, by proteolytic cleavage.C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s.

Gene Full Name: complement C1r

Gen-ID: 715

UniProt: P00736

Pathways: Komplementsystem