C1S ELISA Kit

Produktnummer ABIN8055505

Das Human C1S ELISA Kit (ABIN8055505) ist ein Colorimetric ELISA Kit zur Detektion von Human C1S.

Kurzübersicht für C1S ELISA Kit (ABIN8055505)

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Bindungsspezifität: AA 16-688

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum, Urine

Produktdetails C1S ELISA Kit

Untere Nachweisgrenze: 0.78 ng/mL

Verwendungszweck: Sandwich Human C1S ELISA Kit to quantitate Human C1S in cell culture supernatants, serum, plasma (heparin, EDTA or citrate) , urine, human milk.

Analytische Methode: Quantitative

Spezifität: Expression system for standard: NS0
Immunogen sequence: E16-D688

Capture antibody and Detection antibody: A monoclonal antibody from mouse|a biotinylated detection polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 0.1 ng/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.2%, 6.7%, 4.5%
Inter-Assay Precision (CV%): 5.9%, 7.1%, 4.3%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Details zu C1S

Target: C1S (Complement Component 1, S Subcomponent (C1S))

Andere Bezeichnung: C1S

Hintergrund:

Background: Complement component 1s is a protein involved in the complement system. It is mapped to 12p13.31. This gene encodes a serine protease, which is a major constituent of the human complement subcomponent C1. C1s associates with two other complement components C1r and C1q in order to yield the first component of the serum complement system. Defects in this gene are the cause of selective C1s deficiency.

Gene Full Name: complement C1s

Gen-ID: 716

UniProt: P09871

Pathways: Komplementsystem