IL-5 ELISA Kit

Produktnummer ABIN7849899

Ratte IL-5 ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte IL-5.

Kurzübersicht für IL-5 ELISA Kit (ABIN7849899)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6 pg/mL - 1000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

Produktdetails IL-5 ELISA Kit

Untere Nachweisgrenze: 15.6 pg/mL

Verwendungszweck: For quantitative detection of IL-5 in Rat serum, plasma, tissue lysatesor cell culture supernatants.

Analytische Methode: Quantitative

Sensitivität: < 10 pg/mL

Benötigtes Material:

1. 37 °C incubator
2. Microplate reader (wavelength: 450 nm)
3. Precise pipette and disposable pipette tips
4. Automated plate washer
5. ELISA shaker
6. 1.5 mL of Eppendorf tubes
7. Plate cover
8. Absorbent filter papers
9. Plastic or glass container with volume of above 1 L

Anwendungsinformationen

Plattentyp: Pre-coated

Aufbereitung der Reagenzien:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37 °C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8°C for 6 months, or at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Andere Bezeichnung: IL-5

Pathways: JAK-STAT Signalweg, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour