Kallikrein 8 ELISA Kit

Produktnummer ABIN7833049

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human Kallikrein 8.

Kurzübersicht für Kallikrein 8 ELISA Kit (ABIN7833049)

Target: Kallikrein 8 (KLK8)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 781 pg/mL - 50000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum

Produktdetails Kallikrein 8 ELISA Kit

Untere Nachweisgrenze: 781 pg/mL

Verwendungszweck: Sandwich ELISA for quantitative detection of Human Kallikrein 8 concentrations.

Analytische Methode: Quantitative

Sensitivität: 244 pg/mL

Bestandteile:

• Coated 96-well Plate
• Standard
• Biotin-Labeled Detection Antibody (100X)
• Streptavidin-HRP (100X)
• Standard/Sample Diluent
• Detection Antibody Diluent
• Streptavidin-HRP Diluent
• Wash Buffer (20X)
• TMB Substrate Solution
• Stop Solution
• Plate Adhesive Strips
• Technical Manual

Benötigtes Material:

1. Microplate reader capable of measuring absorbance at 450 nm.
2. Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
3. Adjustable 1-25 ml pipettes for reagent preparation.
4. 100 ml and 1 liter graduated cylinders.
5. Absorbent paper.
6. Distilled or deionized water.
7. Computer and software for ELISA data analysis.
8. Tubes to prepare standard or sample dilutions.

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

• Prepare all reagents, samples and standards
• Add 100 µL Standard or Sample
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Biotin-Labeled Detection Antibody Working Solution
• Wash plate 3 times with Wash Buffer Working Solution
• Add 100 µL Steptavidin-HRP Working Solution
• Wash plate 5 times with Wash Buffer Working Solution
• Add 100 µL TMB Substrat Solution
• Add 100 µL Stop Solution
• Read the plate at 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Kallikrein 8

Target: Kallikrein 8 (KLK8)

Andere Bezeichnung: KLK8

Hintergrund: Alternative Names: NRPN, PRSS19, TADG14, Kallikrein-8, hK8, Neuropsin, NP, Ovasin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein

Gen-ID: 11202

UniProt: O60259

Pathways: Komplementsystem