NLRP3 ELISA Kit

Produktnummer ABIN7832872

Human NLRP3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human NLRP3.

Kurzübersicht für NLRP3 ELISA Kit (ABIN7832872)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Serum

Güteklasse: QuickTest

Produktdetails NLRP3 ELISA Kit

Verwendungszweck: FastELISA NLRP3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human NLRP3 (NLR family pyrin domain containing 3) in Serum, plasma.

Analytische Methode: Quantitative

Spezifität: This assay is highly specific for detection of Human NLRP3. No significant cross-reactivity or interference between Human NLRP3 and analogues was observed.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Haltbarkeit: 6 months

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NLRP3

Hintergrund:

Synonyms: AGTAVPRL|AII|AII/AVP|AVP|C1orf7|CIAS1|CLR1.1|FCAS|FCU|FLJ95925|MWS|NALP3|PYPAF1

Gene Description: NLR family, pyrin domain containing 3

Gen-ID: 114548

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome