PLA2G7 ELISA Kit

Produktnummer ABIN7832867

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human PLA2G7.

Kurzübersicht für PLA2G7 ELISA Kit (ABIN7832867)

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Serum

Güteklasse: QuickTest

Produktdetails PLA2G7 ELISA Kit

Verwendungszweck: FastELISA PLA2G7 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human PLA2G7 (Lipoprotein-associated phospholipase A2) in Serum, plasma.

Analytische Methode: Quantitative

Spezifität: This assay is highly specific for detection of Human PLA2G7. No significant cross-reactivity or interference between Human PLA2G7 and analogues was observed.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Haltbarkeit: 6 months

Details zu PLA2G7

Target: PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))

Andere Bezeichnung: PLA2G7

Hintergrund:

Synonyms: LDL-PLA2|PAFAH

Gene Description: phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma)

Gen-ID: 7941

Pathways: Peptide Hormone Metabolism