FSH ELISA Kit

Produktnummer ABIN7832860

Human FSH ELISA Kit, Colorimetric Assay für die Quantifizierung von Human FSH.

Kurzübersicht für FSH ELISA Kit (ABIN7832860)

Target: FSH (Follicle-Stimulating Hormone (FSH))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Proben: Plasma, Serum

Güteklasse: QuickTest

Produktdetails FSH ELISA Kit

Verwendungszweck: FastELISA FSH (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human FSH (Follicle stimulating hormone) in Serum, plasma.

Analytische Methode: Quantitative

Spezifität: This assay is highly specific for detection of Human FSH. No significant cross-reactivity or interference between Human FSH and analogues was observed.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Haltbarkeit: 6 months

Details zu FSH

Target: FSH (Follicle-Stimulating Hormone (FSH))

Andere Bezeichnung: FSH

Substanzklasse: Hormone

Pathways: Peptide Hormone Metabolism, Chromatin Binding