DDIT3 ELISA Kit

Produktnummer ABIN7831015

Human DDIT3 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human DDIT3.

Kurzübersicht für DDIT3 ELISA Kit (ABIN7831015)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Produktdetails DDIT3 ELISA Kit

Verwendungszweck: DDIT3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of DDIT3 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Andere Bezeichnung: DDIT3

Hintergrund:

Synonyms: CEBPZ|CHOP|CHOP10|GADD153|MGC4154

Gene Description: DNA-damage-inducible transcript 3

Gen-ID: 1649

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis