TUBA1A ELISA Kit

Produktnummer ABIN7831010

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human TUBA1A.

Kurzübersicht für TUBA1A ELISA Kit (ABIN7831010)

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails TUBA1A ELISA Kit

Verwendungszweck: TUBA1A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Tubulin alpha expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu TUBA1A

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Andere Bezeichnung: TUBA1A

Hintergrund:

Synonyms: B-ALPHA-1|FLJ25113|LIS3|TUBA3

Gene Description: tubulin, alpha 1a

Gen-ID: 7846

UniProt: Q71U36

Pathways: Microtubule Dynamics, M Phase