PDE4C ELISA Kit

Produktnummer ABIN7830993

Das Human PDE4C ELISA Kit (ABIN7830993) ist ein Colorimetric ELISA Kit zur Detektion von Human PDE4C.

Kurzübersicht für PDE4C ELISA Kit (ABIN7830993)

Target: PDE4C (phosphodiesterase 4C, CAMP-Specific (PDE4C))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PDE4C ELISA Kit

Verwendungszweck: PDE4D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PDE4D expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu PDE4C

Target: PDE4C (phosphodiesterase 4C, CAMP-Specific (PDE4C))

Andere Bezeichnung: PDE4C

Hintergrund:

Synonyms: DPDE1|MGC126222

Gene Description: phosphodiesterase 4C, cAMP-specific (phosphodiesterase E1 dunce homolog, Drosophila)

Gen-ID: 5143

UniProt: Q08493

Pathways: Negative Regulation of Hormone Secretion, cAMP Metabolic Process