NCF1 ELISA Kit

Produktnummer ABIN7830989

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human NCF1.

Kurzübersicht für NCF1 ELISA Kit (ABIN7830989)

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NCF1 ELISA Kit

Verwendungszweck: NCF1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of p47 phox expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu NCF1

Target: NCF1 (Neutrophil Cytosol Factor 1 (NCF1))

Andere Bezeichnung: NCF1

Hintergrund:

Synonyms: FLJ79451|NCF1A|NOXO2|SH3PXD1A|p47phox

Gene Description: neutrophil cytosolic factor 1

Gen-ID: 653361

UniProt: P14598

Pathways: PI3K-Akt Signalweg