NUB1 ELISA Kit

Produktnummer ABIN7830987

Das Human NUB1 ELISA Kit (ABIN7830987) ist ein Colorimetric ELISA Kit zur Detektion von Human NUB1.

Kurzübersicht für NUB1 ELISA Kit (ABIN7830987)

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails NUB1 ELISA Kit

Verwendungszweck: NUB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NYREN18 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for six months.

Details zu NUB1

Target: NUB1 (Negative Regulator of Ubiquitin-Like Proteins 1 (NUB1))

Andere Bezeichnung: NUB1

Hintergrund:

Synonyms: BS4|NUB1L|NYREN18

Gene Description: negative regulator of ubiquitin-like proteins 1

Gen-ID: 51667

UniProt: Q9Y5A7