VAV1 ELISA Kit

Produktnummer ABIN7830934

Human VAV1 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human VAV1.

Kurzübersicht für VAV1 ELISA Kit (ABIN7830934)

Target: VAV1 (Vav 1 Oncogene (VAV1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails VAV1 ELISA Kit

Verwendungszweck: VAV1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of VAV1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu VAV1

Target: VAV1 (Vav 1 Oncogene (VAV1))

Andere Bezeichnung: VAV1

Hintergrund:

Synonyms: VAV

Gene Description: vav 1 guanine nucleotide exchange factor

Gen-ID: 7409

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, BCR Signaling