SYK ELISA Kit

Produktnummer ABIN7830906

Das Human SYK ELISA Kit (ABIN7830906) ist ein Colorimetric ELISA Kit zur Detektion von Human SYK.

Kurzübersicht für SYK ELISA Kit (ABIN7830906)

Target: SYK (Spleen tyrosine Kinase (SYK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails SYK ELISA Kit

Verwendungszweck: SYK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SYK expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Andere Bezeichnung: SYK

Hintergrund:

Synonyms: DKFZp313N1010|FLJ25043|FLJ37489

Gene Description: spleen tyrosine kinase

Gen-ID: 6850

Pathways: Fc-epsilon Rezeptor Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling