SGK1 ELISA Kit

Produktnummer ABIN7830887

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human SGK1.

Kurzübersicht für SGK1 ELISA Kit (ABIN7830887)

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails SGK1 ELISA Kit

Verwendungszweck: SGK1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of SGK1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu SGK1

Target: SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))

Andere Bezeichnung: SGK1

Hintergrund:

Synonyms: SGK

Gene Description: serum/glucocorticoid regulated kinase 1

Gen-ID: 6446

Pathways: MAPK Signalweg, Notch Signalweg, Steroid Hormone Mediated Signaling Pathway