PDGFB ELISA Kit

Produktnummer ABIN7830836

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human PDGFB.

Kurzübersicht für PDGFB ELISA Kit (ABIN7830836)

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PDGFB ELISA Kit

Verwendungszweck: PDGFB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PDGFB expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PDGFB

Target: PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))

Andere Bezeichnung: PDGFB

Hintergrund:

Synonyms: FLJ12858|PDGF2|SIS|SSV|c-sis

Gene Description: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)

Gen-ID: 5155

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling