PPP1R12A ELISA Kit

Produktnummer ABIN7830816

Das Human PPP1R12A ELISA Kit (ABIN7830816) ist ein Colorimetric ELISA Kit zur Detektion von Human PPP1R12A.

Kurzübersicht für PPP1R12A ELISA Kit (ABIN7830816)

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PPP1R12A ELISA Kit

Verwendungszweck: PPP1R12A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PPP1R12A expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PPP1R12A

Target: PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))

Andere Bezeichnung: PPP1R12A

Hintergrund:

Synonyms: MBS|MGC133042|MYPT1

Gene Description: protein phosphatase 1, regulatory (inhibitor) subunit 12A

Gen-ID: 4659

Pathways: M Phase