XIAP ELISA Kit

Produktnummer ABIN7830783

Das Human XIAP ELISA Kit (ABIN7830783) ist ein Colorimetric ELISA Kit zur Detektion von Human XIAP.

Kurzübersicht für XIAP ELISA Kit (ABIN7830783)

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails XIAP ELISA Kit

Verwendungszweck: XIAP (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of XIAP expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu XIAP

Target: XIAP (X-Linked Inhibitor of Apoptosis (XIAP))

Andere Bezeichnung: XIAP

Hintergrund:

Synonyms: API3|BIRC4|ILP1|MIHA|XLP2

Gene Description: X-linked inhibitor of apoptosis

Gen-ID: 331

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Transition Metal Ion Homeostasis