MMP2 ELISA Kit

Produktnummer ABIN7830776

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human MMP2.

Kurzübersicht für MMP2 ELISA Kit (ABIN7830776)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails MMP2 ELISA Kit

Verwendungszweck: MMP2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MMP2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: MMP2

Hintergrund:

Synonyms: CLG4|CLG4A|MMP-II|MONA|TBE-1

Gene Description: matrix metallopeptidase 2 (gelatinase A, 72 kDa gelatinase, 72 kDa type IV collagenase)

Gen-ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response