MEF2A ELISA Kit

Produktnummer ABIN7830756

Das Human MEF2A ELISA Kit (ABIN7830756) ist ein Colorimetric ELISA Kit zur Detektion von Human MEF2A.

Kurzübersicht für MEF2A ELISA Kit (ABIN7830756)

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails MEF2A ELISA Kit

Verwendungszweck: MEF2A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MEF2A expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu MEF2A

Target: MEF2A (Myocyte Enhancer Factor 2A (MEF2A))

Andere Bezeichnung: MEF2A

Hintergrund:

Synonyms: ADCAD1|RSRFC4|RSRFC9

Gene Description: myocyte enhancer factor 2A

Gen-ID: 4205

UniProt: Q02078

Pathways: Neurotrophin Signalübertragung, Activation of Innate immune Response, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades