MCM2 ELISA Kit

Produktnummer ABIN7830751

Human MCM2 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human MCM2.

Kurzübersicht für MCM2 ELISA Kit (ABIN7830751)

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails MCM2 ELISA Kit

Verwendungszweck: MCM2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MCM2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu MCM2

Target: MCM2 (Minichromosome Maintenance Complex Component 2 (MCM2))

Andere Bezeichnung: MCM2

Hintergrund:

Synonyms: BM28|CCNL1|CDCL1|D3S3194|KIAA0030|MGC10606|MITOTIN|cdc19

Gene Description: minichromosome maintenance complex component 2

Gen-ID: 4171

UniProt: P49736

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA