MAPKAP Kinase 5 ELISA Kit

Produktnummer ABIN7830747

Das Human MAPKAP Kinase 5 ELISA Kit (ABIN7830747) ist ein Colorimetric ELISA Kit zur Detektion von Human MAPKAP Kinase 5.

Kurzübersicht für MAPKAP Kinase 5 ELISA Kit (ABIN7830747)

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails MAPKAP Kinase 5 ELISA Kit

Verwendungszweck: MAPKAPK5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAPKAPK5 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu MAPKAP Kinase 5

Target: MAPKAP Kinase 5 (MAPKAPK5) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 5 (MAPKAPK5))

Andere Bezeichnung: MAPKAPK5

Hintergrund:

Synonyms: PRAK

Gene Description: mitogen-activated protein kinase-activated protein kinase 5

Gen-ID: 8550

UniProt: Q8IW41

Pathways: MAPK Signalweg