LCK ELISA Kit

Produktnummer ABIN7830735

Das Human LCK ELISA Kit (ABIN7830735) ist ein Colorimetric ELISA Kit zur Detektion von Human LCK.

Kurzübersicht für LCK ELISA Kit (ABIN7830735)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails LCK ELISA Kit

Verwendungszweck: LCK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LCK expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Andere Bezeichnung: LCK

Hintergrund:

Synonyms: YT16|p56lck|pp58lck

Gene Description: lymphocyte-specific protein tyrosine kinase

Gen-ID: 3932

UniProt: P06239

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling