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LCK ELISA Kit

LCK Reaktivität: Human pTyr Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886174
  • Target Alle LCK ELISA Kits anzeigen
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Bindungsspezifität
    pTyr
    Reaktivität
    • 6
    • 4
    • 4
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Custom Human Phosphotyrosine LCK ELISA Kit. This assay semi-quantitatively measures phosphotyrosine LCK in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-LCK.
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product LCK ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle LCK ELISA Kits anzeigen
    LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
    Andere Bezeichnung
    LCK (LCK Produkte)
    Synonyme
    zgc:136695 ELISA Kit, LCK ELISA Kit, Hck-3 ELISA Kit, Lsk ELISA Kit, Lskt ELISA Kit, p56 ELISA Kit, p56Lck ELISA Kit, LSK ELISA Kit, YT16 ELISA Kit, p56lck ELISA Kit, pp58lck ELISA Kit, P56LCK ELISA Kit, tkl ELISA Kit, Lck1 ELISA Kit, Lcktkr ELISA Kit, LCK proto-oncogene, Src family tyrosine kinase ELISA Kit, lymphocyte protein tyrosine kinase ELISA Kit, lck ELISA Kit, LCK ELISA Kit, Lck ELISA Kit
    Gen-ID
    3932
    UniProt
    P06239
    Pathways
    T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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