Cytokeratin 18 ELISA Kit

Produktnummer ABIN7830721

Human Cytokeratin 18 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Cytokeratin 18.

Kurzübersicht für Cytokeratin 18 ELISA Kit (ABIN7830721)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Cytokeratin 18 ELISA Kit

Verwendungszweck: KRT18 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of KRT18 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: KRT18

Hintergrund:

Synonyms: CYK18|K18

Gene Description: keratin 18

Gen-ID: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose