Glucagon ELISA Kit

Produktnummer ABIN7830676

Human Glucagon ELISA Kit, Colorimetric Assay für die Quantifizierung von Human Glucagon.

Kurzübersicht für Glucagon ELISA Kit (ABIN7830676)

Target: Glucagon (GCG)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Glucagon ELISA Kit

Verwendungszweck: GCG (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GCG expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Glucagon

Target: Glucagon (GCG)

Andere Bezeichnung: GCG

Hintergrund:

Synonyms: GLP1|GLP2|GRPP

Gene Description: glucagon

Gen-ID: 2641

UniProt: P01275

Pathways: Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Negative Regulation of intrinsic apoptotic Signaling