GATA4 ELISA Kit

Produktnummer ABIN7830673

Human GATA4 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human GATA4.

Kurzübersicht für GATA4 ELISA Kit (ABIN7830673)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails GATA4 ELISA Kit

Verwendungszweck: GATA4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of GATA4 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Andere Bezeichnung: GATA4

Hintergrund:

Synonyms: MGC126629

Gene Description: GATA binding protein 4

Gen-ID: 2626

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis