FYN ELISA Kit

Produktnummer ABIN7830663

Das Human FYN ELISA Kit (ABIN7830663) ist ein Colorimetric ELISA Kit zur Detektion von Human FYN.

Kurzübersicht für FYN ELISA Kit (ABIN7830663)

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails FYN ELISA Kit

Verwendungszweck: FYN (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FYN expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu FYN

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Andere Bezeichnung: FYN

Hintergrund:

Synonyms: MGC45350|SLK|SYN

Gene Description: FYN oncogene related to SRC, FGR, YES

Gen-ID: 2534

UniProt: P06241

Pathways: JAK-STAT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling