FRS2 ELISA Kit

Produktnummer ABIN7830662

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human FRS2.

Kurzübersicht für FRS2 ELISA Kit (ABIN7830662)

Target: FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails FRS2 ELISA Kit

Verwendungszweck: FRS2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FRS2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu FRS2

Target: FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))

Andere Bezeichnung: FRS2

Hintergrund:

Synonyms: FRS2A|FRS2alpha|SNT|SNT-1|SNT1

Gene Description: fibroblast growth factor receptor substrate 2

Gen-ID: 10818

UniProt: Q8WU20

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Signaling Events mediated by VEGFR1 and VEGFR2