Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

FRS2 ELISA Kit

FRS2 Reaktivität: Human, Maus, Ratte pTyr Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Produktnummer ABIN6810504
  • Target Alle FRS2 ELISA Kits anzeigen
    FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
    Bindungsspezifität
    pTyr
    Reaktivität
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human, Mouse and Rat Phosphotyrosine FRS2 ELISA Kit. This assay semi-quantitatively measures tyrosine-phosphorylated FRS2 in cell lysate samples.
    Proben
    Cell Samples, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    This ELISA kit recognizes human FRS2 phosphotyrosine.
    Produktmerkmale
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Biotinylated Anti-Phosphotyrosine Antibody
    • Stop Solution
    • Assay Diluent(s)
    • Positive Control Sample
    • Lysis Buffer
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Zu unserem meistverkauften FRS2 ELISA Kit
    Top Product
    Discover our top product FRS2 ELISA Kit
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle FRS2 ELISA Kits anzeigen
    FRS2 (Fibroblast Growth Factor Receptor Substrate 2 (FRS2))
    Andere Bezeichnung
    FRS2 (FRS2 Produkte)
    Synonyme
    frs2 ELISA Kit, wu:fd21h10 ELISA Kit, zgc:91973 ELISA Kit, FRS2A ELISA Kit, FRS2alpha ELISA Kit, SNT ELISA Kit, SNT-1 ELISA Kit, SNT1 ELISA Kit, 4732458E18 ELISA Kit, C330018A15Rik ELISA Kit, Frs2alpha ELISA Kit, fibroblast growth factor receptor substrate 2 ELISA Kit, fibroblast growth factor receptor substrate 2b ELISA Kit, FRS2 ELISA Kit, frs2b ELISA Kit, Frs2 ELISA Kit
    Gen-ID
    10818
    UniProt
    Q8WU20, Q8C180
    Pathways
    Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Signaling Events mediated by VEGFR1 and VEGFR2
Sie sind hier:
Kundenservice