FOXO4 ELISA Kit

Produktnummer ABIN7830660

Das Human FOXO4 ELISA Kit (ABIN7830660) ist ein Colorimetric ELISA Kit zur Detektion von Human FOXO4.

Kurzübersicht für FOXO4 ELISA Kit (ABIN7830660)

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails FOXO4 ELISA Kit

Verwendungszweck: FOXO4 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FOXO4 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu FOXO4

Target: FOXO4 (Forkhead Box O4 (FOXO4))

Andere Bezeichnung: FOXO4

Hintergrund:

Synonyms: AFX|AFX1|MGC120490|MLLT7

Gene Description: forkhead box O4

Gen-ID: 4303

UniProt: P98177

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation