FASL ELISA Kit

Produktnummer ABIN7830645

Das Human FASL ELISA Kit (ABIN7830645) ist ein Colorimetric ELISA Kit zur Detektion von Human FASL.

Kurzübersicht für FASL ELISA Kit (ABIN7830645)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails FASL ELISA Kit

Verwendungszweck: FASLG (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of FASLG expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Andere Bezeichnung: FASLG

Hintergrund:

Synonyms: APT1LG1|CD178|CD95L|FASL|TNFSF6

Gene Description: Fas ligand (TNF superfamily, member 6)

Gen-ID: 356

UniProt: P48023

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity