ERCC1 ELISA Kit

Produktnummer ABIN7830633

Das Human ERCC1 ELISA Kit (ABIN7830633) ist ein Colorimetric ELISA Kit zur Detektion von Human ERCC1.

Kurzübersicht für ERCC1 ELISA Kit (ABIN7830633)

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails ERCC1 ELISA Kit

Verwendungszweck: ERCC1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ERCC1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu ERCC1

Target: ERCC1 (Excision Repair Cross Complementing Polypeptide-1 (ERCC1))

Andere Bezeichnung: ERCC1

Hintergrund:

Synonyms: COFS4|UV20

Gene Description: excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence)

Gen-ID: 2067

UniProt: P07992

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response