EPH Receptor B2 ELISA Kit

Produktnummer ABIN7830629

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human EPH Receptor B2.

Kurzübersicht für EPH Receptor B2 ELISA Kit (ABIN7830629)

Target: EPH Receptor B2 (EPHB2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails EPH Receptor B2 ELISA Kit

Verwendungszweck: EPHB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPHB2 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EPHB2

Hintergrund:

Synonyms: CAPB|DRT|EPHT3|ERK|Hek5|MGC87492|PCBC|Tyro5

Gene Description: EPH receptor B2

Gen-ID: 2048

UniProt: P29323

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine