EPH Receptor B1 ELISA Kit

Produktnummer ABIN7830627

Das Human EPH Receptor B1 ELISA Kit (ABIN7830627) ist ein Colorimetric ELISA Kit zur Detektion von Human EPH Receptor B1.

Kurzübersicht für EPH Receptor B1 ELISA Kit (ABIN7830627)

Target: EPH Receptor B1 (EPHB1)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails EPH Receptor B1 ELISA Kit

Verwendungszweck: EPHB1/EPHB2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPHB1/EPHB2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu EPH Receptor B1

Target: EPH Receptor B1 (EPHB1)

Andere Bezeichnung: EPHB1

Hintergrund:

Synonyms: ELK|EPHT2|FLJ37986|Hek6|NET

Gene Description: EPH receptor B1

Gen-ID: 2047

UniProt: P54762

Pathways: RTK Signalweg