EPH Receptor A5 ELISA Kit

Produktnummer ABIN7830625

Human EPH Receptor A5 ELISA Kit, Colorimetric Assay für die Quantifizierung von Human EPH Receptor A5.

Kurzübersicht für EPH Receptor A5 ELISA Kit (ABIN7830625)

Target: EPH Receptor A5 (EPHA5)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails EPH Receptor A5 ELISA Kit

Verwendungszweck: EPHA5 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EPHA5 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu EPH Receptor A5

Target: EPH Receptor A5 (EPHA5)

Andere Bezeichnung: EPHA5

Hintergrund:

Synonyms: CEK7|EHK1|HEK7|TYRO4

Gene Description: EPH receptor A5

Gen-ID: 2044

UniProt: P54756

Pathways: RTK Signalweg