CD25 ELISA Kit

Produktnummer ABIN7830603

Das Human CD25 ELISA Kit (ABIN7830603) ist ein Colorimetric ELISA Kit zur Detektion von Human CD25.

Kurzübersicht für CD25 ELISA Kit (ABIN7830603)

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CD25 ELISA Kit

Verwendungszweck: IL2RA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IL2RA expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CD25

Target: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Andere Bezeichnung: IL2RA

Hintergrund:

Synonyms: CD25|IDDM10|IL2R|TCGFR

Gene Description: interleukin 2 receptor, alpha

Gen-ID: 3559

UniProt: P01589

Pathways: JAK-STAT Signalweg, Growth Factor Binding, Activated T Cell Proliferation