IGFBP3 ELISA Kit
Kurzübersicht für IGFBP3 ELISA Kit (ABIN7830596)
Target
Alle IGFBP3 ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Applikation
Proben
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Verwendungszweck
- IGFBP3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of IGFBP3 expression in cultured cells.
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Analytische Methode
- Qualitative
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Kreuzreaktivität
- Maus
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Applikationshinweise
- Optimal working dilution should be determined by the investigator.
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Plattentyp
- Uncoated
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Protokoll
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store the kit at 4°C.
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- IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))
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Andere Bezeichnung
- IGFBP3
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Hintergrund
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Synonyms: BP-53|IBP3
Gene Description: insulin-like growth factor binding protein 3
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Gen-ID
- 3486
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UniProt
- P17936
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Pathways
- Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Growth Factor Binding
Target Alle IGFBP3 ELISA Kits anzeigen
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