H2AFX ELISA Kit

Produktnummer ABIN7830577

Human H2AFX ELISA Kit, Colorimetric Assay für die Quantifizierung von Human H2AFX.

Kurzübersicht für H2AFX ELISA Kit (ABIN7830577)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails H2AFX ELISA Kit

Verwendungszweck: H2AFX (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of H2AFX expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung: H2AFX

Hintergrund:

Synonyms: H2A.X|H2A/X|H2AX

Gene Description: H2A histone family, member X

Gen-ID: 3014

UniProt: P16104

Pathways: Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus