Cyclin E2 ELISA Kit

Produktnummer ABIN7830543

Das Human Cyclin E2 ELISA Kit (ABIN7830543) ist ein Colorimetric ELISA Kit zur Detektion von Human Cyclin E2.

Kurzübersicht für Cyclin E2 ELISA Kit (ABIN7830543)

Target: Cyclin E2 (CCNE2)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Cyclin E2 ELISA Kit

Verwendungszweck: CCNE2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CCNE2 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Cyclin E2

Target: Cyclin E2 (CCNE2)

Andere Bezeichnung: CCNE2

Hintergrund:

Synonyms: CYCE2

Gene Description: cyclin E2

Gen-ID: 9134

UniProt: O96020

Pathways: Zellzyklus, Mitotic G1-G1/S Phases