C5AR1 ELISA Kit

Produktnummer ABIN7830489

Das Human C5AR1 ELISA Kit (ABIN7830489) ist ein Colorimetric ELISA Kit zur Detektion von Human C5AR1.

Kurzübersicht für C5AR1 ELISA Kit (ABIN7830489)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails C5AR1 ELISA Kit

Verwendungszweck: C5AR1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of C5AR1 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung: C5AR1

Hintergrund:

Synonyms: C5A|C5AR|C5R1|CD88

Gene Description: complement component 5a receptor 1

Gen-ID: 728

UniProt: P21730

Pathways: Komplementsystem