CD247 ELISA Kit

Produktnummer ABIN7830483

Das Human CD247 ELISA Kit (ABIN7830483) ist ein Colorimetric ELISA Kit zur Detektion von Human CD247.

Kurzübersicht für CD247 ELISA Kit (ABIN7830483)

Target: CD247 (CD247 Molecule (CD247))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CD247 ELISA Kit

Verwendungszweck: CD247 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CD247 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CD247

Target: CD247 (CD247 Molecule (CD247))

Andere Bezeichnung: CD247

Hintergrund:

Synonyms: CD3-ZETA|CD3H|CD3Q|CD3Z|T3Z|TCRZ

Gene Description: CD247 Molecule

Gen-ID: 919

UniProt: P20963

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway