Caspase 10 ELISA Kit

Produktnummer ABIN7830468

Das Human Caspase 10 ELISA Kit (ABIN7830468) ist ein Colorimetric ELISA Kit zur Detektion von Human Caspase 10.

Kurzübersicht für Caspase 10 ELISA Kit (ABIN7830468)

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails Caspase 10 ELISA Kit

Verwendungszweck: CASP10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CASP10 expression in cultured cells.

Analytische Methode: Qualitative

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu Caspase 10

Target: Caspase 10 (CASP10) (Caspase 10, Apoptosis-Related Cysteine Peptidase (CASP10))

Andere Bezeichnung: CASP10

Hintergrund:

Synonyms: ALPS2|FLICE2|MCH4

Gene Description: caspase 10, apoptosis-related cysteine peptidase

Gen-ID: 843

UniProt: Q92851

Pathways: Apoptose, Caspase Kaskade in der Apoptose