CAMK2B ELISA Kit

Produktnummer ABIN7830463

Human CAMK2B ELISA Kit, Colorimetric Assay für die Quantifizierung von Human CAMK2B.

Kurzübersicht für CAMK2B ELISA Kit (ABIN7830463)

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails CAMK2B ELISA Kit

Verwendungszweck: CAMK2B/CAMK2G/CAMK2D (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CAMK2B/CAMK2G/CAMK2D expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Andere Bezeichnung: CAMK2B

Hintergrund:

Synonyms: CAM2|CAMK2|CAMKB|MGC29528

Gene Description: calcium/calmodulin-dependent protein kinase II beta

Gen-ID: 816

UniProt: Q13554

Pathways: WNT Signalweg, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity