APC ELISA Kit

Produktnummer ABIN7830419

Dieses Colorimetric ELISA kit dient der quantitativen Messung von Human APC.

Kurzübersicht für APC ELISA Kit (ABIN7830419)

Target: APC (Adenomatous Polyposis Coli (APC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails APC ELISA Kit

Verwendungszweck: APC (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of APC expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu APC

Target: APC (Adenomatous Polyposis Coli (APC))

Andere Bezeichnung: APC

Hintergrund:

Synonyms: BTPS2|DP2|DP2.5|DP3|GS

Gene Description: adenomatous polyposis coli

Gen-ID: 324

UniProt: P25054

Pathways: WNT Signalweg, Stem Cell Maintenance, Cell-Cell Junction Organization