PRKAB1 ELISA Kit

Produktnummer ABIN7830411

Das Human PRKAB1 ELISA Kit (ABIN7830411) ist ein Colorimetric ELISA Kit zur Detektion von Human PRKAB1.

Kurzübersicht für PRKAB1 ELISA Kit (ABIN7830411)

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails PRKAB1 ELISA Kit

Verwendungszweck: PRKAB1 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of PRKAB1 expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Andere Bezeichnung: PRKAB1

Hintergrund:

Synonyms: AMPK|HAMPKb|MGC17785

Gene Description: protein kinase, AMP-activated, beta 1 non-catalytic subunit

Gen-ID: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, Warburg Effekt