14-3-3 zeta ELISA Kit

Produktnummer ABIN7830382

Human 14-3-3 zeta ELISA Kit, Colorimetric Assay für die Quantifizierung von Human 14-3-3 zeta.

Kurzübersicht für 14-3-3 zeta ELISA Kit (ABIN7830382)

Target: 14-3-3 zeta (YWHAZ)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails 14-3-3 zeta ELISA Kit

Verwendungszweck: YWHAZ/YWHAB (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of YWHAZ/YWHAB expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu 14-3-3 zeta

Target: 14-3-3 zeta (YWHAZ)

Andere Bezeichnung: YWHAZ

Hintergrund:

Synonyms: KCIP-1|MGC111427|MGC126532|MGC138156

Gene Description: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide

Gen-ID: 7534

UniProt: P63104

Pathways: Apoptose, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location